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bbsi digested cas9  (Addgene inc)


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    Addgene inc bbsi digested cas9
    Bbsi Digested Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbsi digested cas9/product/Addgene inc
    Average 97 stars, based on 427 article reviews
    bbsi digested cas9 - by Bioz Stars, 2026-04
    97/100 stars

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    CRISPR/Cas9 gene‐edited erythroblasts surface antigen presentation. Complete loss of RhAG, RhD/CE, GPB protein expression was confirmed for RhAG, RhD/CE (A) and GPB (B) erythroblasts. Anti‐U was used to confirm GPB deletion. Kell glycoprotein expression was evaluated as a proxy for XK knockout erythroblasts. Kell expression (C) was considerably diminished consistently with the lack of Kx expression. KO, knockout

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Generation of ‘designer erythroblasts’ lacking one or more blood group systems from CRISPR/Cas9 gene‐edited human‐induced pluripotent stem cells

    doi: 10.1111/jcmm.16872

    Figure Lengend Snippet: CRISPR/Cas9 gene‐edited erythroblasts surface antigen presentation. Complete loss of RhAG, RhD/CE, GPB protein expression was confirmed for RhAG, RhD/CE (A) and GPB (B) erythroblasts. Anti‐U was used to confirm GPB deletion. Kell glycoprotein expression was evaluated as a proxy for XK knockout erythroblasts. Kell expression (C) was considerably diminished consistently with the lack of Kx expression. KO, knockout

    Article Snippet: Briefly, cloning was performed by ligating annealed‐guides into BbsI digested GFP‐Cas9 expression vector pX458 (one guide/vector) using a quick ligation kit (New England Biolabs, Ipswich, MA, USA) and transformed into competent E. coli cells (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: CRISPR, Expressing, Knock-Out